Coding

Part:BBa_K2182000

Designed by: David Rosenberg   Group: iGEM16_Northeastern   (2016-10-14)


S. sanguinis H2O forming NADPH Dehydrogenase

This part codes for an enzyme which oxidizes NADH by transferring Hydrogen from NADH to oxygen (O2) molecules, forming NAD+ and water.

Usage and Biology

The Nox gene regenerates NAD+ to balance redox metabolism while reducing oxidative stress, with H2O - forming or H2O2 - forming relatives in many species of prokaryotes and eukaryotes. Nox has also been implicated in biofilm formation of Streptococcus sanguinis. Ge et al. successfully expressed the recombinant nox protein in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Ge X, Yu Y, Zhang M, Chen L, Chen W, Elrami F, Kong F, Kitten T, Xu P. 2016. Involvement of NADH oxidase in competition and endocarditis virulence in Streptococcus sanguinis. Infect Immun 84:1470-1477. doi:10.1128/IAI.01203-15.
Ge X, Shi X, Shi L, Liu J, Stone V, Kong F, et al. 2016. Involvement of NADH Oxidase in Biofilm Formation in Streptococcus sanguinis. PLoS ONE 11(3): e0151142. doi:10.1371/journal.pone.0151142.

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